Instrument Info

BD FACSCalibur cell analyzer

Cell analyzer, Model: BD FACSCalibur, has two lasers (488nm Blue Laser, 633nm Red Laser). It has 4 fluorescence detectors.

  • Excitation Laser  488nm  Blue Laser 
  • Emission Filter  /  fluorochrome example
                     A: 530/30  |  Alexa Fluor 488, FITC, BB515
                     B: 575/26  |  PE, PI,
                     C: 670LP   |  PerCP, PerCP-Cy5.5, BB700
                  
  • Excitation Laser  633nm  Red Laser 
  • Emission Filter  |  fluorochrome example
                    A: 661/16  |  APC, Alexa Fluor 647