CGU Reservation System

Instrument Info

Thermo Attune NxT cell analyzer ( 637nm laser error, can't use APC,APC-Cy7 etc dye ) )

Cell analyzer, Model: ThermoFisher Attune NxT, has four lasers (488nm Blue Laser, 633nm Red Laser, 405nm Violet Laser and 561nm Yellow Laser). It has 14 fluorescence detectors.

Excitation Laser 488nm Blue Laser
Emission Filter | fluorochrome example

BL1: 530/30 | Alexa Fluor 488, FITC, BB515
BL2: 575/26 | PE-Alexa Fluor 610, PE, PE-Texas Red
BL3: 695/40 | PerCP, PE-Cy5.5, PerCP,-Cy5.5, BB700

Excitation Laser 637nm Red Laser
Emission Filter / fluorochrome example

RL1: 670/14 | APC, Alexa Fluor 647
RL2: 720/30 | Alexa Fluor 680, APC-Alexa Fluor 700
RL3: 780/60 | APC-Cy7, APC-Alexa Fluor 750

Excitation Laser 405nm Violet Laser
Emission Filter / fluorochrome example

VL1: 450/40 | Alexa Fluor 405, V450, BV421, Pacific Blue
VL2: 512/25 | Pacific Green
VL3: 603/48 | Pacific Orange, Qdot*605
VL4: 710/50 | Qdot*705

Excitation Laser 561nm Yellow Laser
Emission Filter / fluorochrome example

YL1: 585/16 | PE, PI
YL2: 620/15 | PE-Texas Red, PE-Alexa Fluor 610, 7-AAD
YL3: 695/40 | PE-Cy5.5, PE-Alexa Fluor 700
YL4: 780/60 | PE-Cy7, Qdot*800