特色
多色流式細胞分析儀 Thermo Attune NxT,現備有4支雷射 (488nm Blue Laser、637nm Red Laser、 405nm Violet Laser及 561nm Yellow Laser),設有14個螢光檢測器,可同時激發出14色螢光。
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Excitation Laser 488nm Blue Laser
- Emission Filter | fluorochrome example
BL1: 530/30 | Alexa Fluor 488, FITC, BB515
BL2: 575/26 | PE-Alexa Fluor 610, PE, PE-Texas Red
BL3: 695/40 | PerCP, PE-Cy5.5, PerCP,-Cy5.5, BB700
BL2: 575/26 | PE-Alexa Fluor 610, PE, PE-Texas Red
BL3: 695/40 | PerCP, PE-Cy5.5, PerCP,-Cy5.5, BB700
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Excitation Laser 637nm Red Laser
- Emission Filter | fluorochrome example
RL1: 670/14 | APC, Alexa Fluor 647
RL2: 720/30 | Alexa Fluor 680, APC-Alexa Fluor 700
RL3: 780/60 | APC-Cy7, APC-Alexa Fluor 750
RL3: 780/60 | APC-Cy7, APC-Alexa Fluor 750
- Excitation Laser 405nm Violet Laser
- Emission Filter | fluorochrome example
VL1: 450/40 | Alexa Fluor 405, V450, BV421, Pacific Blue
VL2: 512/25 | Pacific Green
VL3: 603/48 | Pacific Orange, Qdot*605
VL4: 710/50 | Qdot*705
VL2: 512/25 | Pacific Green
VL3: 603/48 | Pacific Orange, Qdot*605
VL4: 710/50 | Qdot*705
- Excitation Laser 561nm Yellow Laser
- Emission Filter | fluorochrome example
YL1: 585/16 | PE, PI
YL2: 620/15 | PE-Texas Red, PE-Alexa Fluor 610, 7-AAD
YL3: 695/40 | PE-Cy5.5, PE-Alexa Fluor 700
YL4: 780/60 | PE-Cy7, Qdot*800
YL2: 620/15 | PE-Texas Red, PE-Alexa Fluor 610, 7-AAD
YL3: 695/40 | PE-Cy5.5, PE-Alexa Fluor 700
YL4: 780/60 | PE-Cy7, Qdot*800