CGU Reservation System

Notice

Only those user who are qualified to operate the instrument by themselves can use this reservation page, and those who need to entrust management to operate or accompany , please make the bookings on the page [Entrust to use ]

How to qualify for flow cytometer ( BD LSR II ) ?

Practical operation and assessment

Accompanied by a person who can operate the machine by himself to practice machine or software. After becoming familiar with the instrument operation process, please ask the manager for assessment and certification requirements to obtain the qualification

If you cannot pass the manager's assessment, please re-familiarize yourself with the instrument operation and re-certify
Apply for Flow cytometry online appointment permission

Please apply for an account in the CGU Reservation System, complete the verification in accordance with the regulations, and wait for the PI to approve, and then start the online appointment

Reservation method :

CGU Reservation System are open every Monday at 09:00 in the morning to next week (Sunday). Reservations must be at least half an hour, and a single appointment cannot exceed four hours.
Unable to make an appointment for the same day schedule or change the bookings that have been reserved for the day. If the day ’s experiment has special reasons to cancel, please call the administrator
If you cannot get on the plane at the agreed time, please cancel the appointment online one day in advance. Otherwise, you will be warned once and charged for the appointment.
If you do not use the instrument at the appointment time and forget to cancel the appointment record for a total of 2 times, the appointment authority for the next month will be cancelled
Schedule up to four times a week (working / popular times), unlimited during non-working times
Please make an appointment with your personal account. It is forbidden to make an appointment with another person's account, and you cannot make an appointment for others.
The user has delayed the use of the machine for some reason and exceeded the time period reserved by him. Please be sure to confirm whether other users continue to make appointments and contact them to inform them by yourself, or ask the administrator to assist in notification. If it is not dealt with, it will affect the use of next user. The next user or the administrator have the right to suspend the program to protect the rights of the person who made the appointment during the period. The administrator can notify PI of the violation and record a warning once.

Operating regulations :

Do not wear gloves when operating computers and instruments; if you have infection doubts, wear gloves as an experimenter, please bring 70-75% alcohol and clean the touched items after the experiment.
Please follow the normal procedures for power on and off and cleaning.
Please fill in the instrument-logbook after use. During working hours, please notify the administrator if there is any abnormality; please explain the abnormal situation in the logbook during non-working hours. Do not adjust the instrument parameters or hardware parts by yourself.
At the end of the experiment, please take away all the experimental items you brought, and jointly maintain a clean and tidy environment of the public instrument. Thank you.

Consumables (prepared by yourself) :

Please prepare 1X PBS (approximately 1L / 1 hour ; 2L / 2 hour ) in advance, and filter the 1X PBS with a 0.22um Filter
FALCON # 352052 Sample Loading Tube
200ul yellow tips or 1000ul blue tips
Nylon Net Filter 30um-50um (For filtering cell clumps to avoid blockage of the machine)

0910 Room has access control. Non- Center personnel enter this room during off hours, please use your own access card to enter or apply to the administrator for a temporary access card. Users who have passed any flow cytometer certification can apply to administrator of Instrumentation Center for access control card settings.

Administrator:

Lu Ya-Fen Miss ; Tel : 03-2118800 ext 5428
E-MAIL : yafenlu@mail.cgu.edu.tw