特色
流式細胞分析儀 BD LSR II,現備有3支雷射 (488nm Blue Laser、633nm Red Laser 及 405nm Violet Laser),設有11個螢光檢測器,可同時激發出11色螢光。
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Excitation Laser 488nm Blue Laser
- Emission Filter | fluorochrome example
A: 530/30 | Alexa Fluor 488, FITC, BB515
B: 575/26 | PE
C: 610/20 | PE-Texas Red
D: 660/20 | PE-Cy5
E: 695/40 | PI, PerCP, PerCP-Cy5.5, BB700, 7-AAD
F: 780/60 | PE-Cy7
B: 575/26 | PE
C: 610/20 | PE-Texas Red
D: 660/20 | PE-Cy5
E: 695/40 | PI, PerCP, PerCP-Cy5.5, BB700, 7-AAD
F: 780/60 | PE-Cy7
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Excitation Laser 633nm Red Laser
- Emission Filter | fluorochrome example
A: 670/30 | APC, Alexa Fluor 647
B: 730/45 | Alexa Fluor 700
C: 780/60 | APC-Cy7, APC-H7
C: 780/60 | APC-Cy7, APC-H7
- Excitation Laser 405nm Violet Laser
- Emission Filter | fluorochrome example
A: 450/50 | Alexa Fluor 405, V450, BV421, PB
B: 525/50 | Alexa Fluor 430, V500, BV510
B: 525/50 | Alexa Fluor 430, V500, BV510