特色
流式細胞分選儀 BD / FACSAriaIIu，現備有4支雷射 (488nm Blue Laser、633nm Red Laser 、 Near UV Laser 及 405nm Violet Laser)，設有9個螢光檢測器，可同時激發出9色螢光。
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Excitation Laser 488nm Blue Laser
- Emission Filter | fluorochrome example
A: 530/30 | Alexa Fluor 488, FITC, BB515
B: 575/26 | PE
C: 610/20 | PE-Texas Red
D: 695/40 | PI, PerCP, PerCP-Cy5.5, BB700
E: 780/60 | PE-Cy7
B: 575/26 | PE
C: 610/20 | PE-Texas Red
D: 695/40 | PI, PerCP, PerCP-Cy5.5, BB700
E: 780/60 | PE-Cy7
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Excitation Laser 633nm Red Laser
- Emission Filter | fluorochrome example
A: 660/20 | APC, Alexa Fluor 647
B: 780/60 | APC-Cy7, APC-H7
- Excitation Laser 375nm Near UV Laser ( 375nm or 405nm 擇一)
- Emission Filter | fluorochrome example
A: 450/40 | DAPI , Hoechst Blue (Cell cycle)
B: 670LP | Hoechst Red (Side population)
B: 670LP | Hoechst Red (Side population)
- Excitation Laser 405nm Violet Laser ( 375nm or 405nm 擇一)
- Emission Filter | fluorochrome example
A: 450/40 | Alexa Fluor 405, V450, BV421, PB
B: 510/50 | Alexa Fluor 430, V500, BV510
B: 510/50 | Alexa Fluor 430, V500, BV510