CGU Reservation System

Instruments

Title	Content	Actions
BD FACSAriaIIu cell sorter Reservable	Cell sorter, Model: BD FACSAriaIIu, has four lasers (488nm Blue Laser, 633nm Red Laser, 375nm Near UV Laser and 405nm Violet Laser ). It has 9 fluorescence detectors. Excitation Laser 488nm Blue Laser *Emission Filter / fluorochrome example* A: 530/30 \| Alexa Fluor 488, FITC, BB515 B: 575/26 \| PE C: 610/20 \| PE-Texas Red D: 695/40 \| PI, PerCP, PerCP-Cy5.5, BB700 E: 780/60 \| PE-Cy7 Excitation Laser 633nm Red Laser *Emission Filter / fluorochrome example* A: 660/20 \| APC, Alexa Fluor 647 B: 780/60 \| APC-Cy7, APC-H7 Excitation Laser 375nm Near UV Laser ( 375nm or 405nm choose one ) *Emission Filter \| fluorochrome example* A: 450/40 \| DAPI , Hoechst Blue (Cell cycle) B: 670LP \| Hoechst Red (Side population) Excitation Laser 405nm Violet Laser ( 375nm or 405nm laser only choose one ) *Emission Filter / fluorochrome example* A: 450/40 \| Alexa Fluor 405, V450, BV421, PB B: 510/50 \| Alexa Fluor 430, V500, BV510	Info Reserve
Thermo Attune NxT cell analyzer ( 637nm laser error, can't use APC,APC-Cy7 etc dye ) ) Reservable	Cell analyzer, Model: ThermoFisher Attune NxT, has four lasers (488nm Blue Laser, 633nm Red Laser, 405nm Violet Laser and 561nm Yellow Laser). It has 14 fluorescence detectors. Excitation Laser 488nm Blue Laser *Emission Filter \| fluorochrome example* BL1: 530/30 \| Alexa Fluor 488, FITC, BB515 BL2: 575/26 \| PE-Alexa Fluor 610, PE, PE-Texas Red BL3: 695/40 \| PerCP, PE-Cy5.5, PerCP,-Cy5.5, BB700 Excitation Laser 637nm Red Laser *Emission Filter / fluorochrome example* RL1: 670/14 \| APC, Alexa Fluor 647 RL2: 720/30 \| Alexa Fluor 680, APC-Alexa Fluor 700 RL3: 780/60 \| APC-Cy7, APC-Alexa Fluor 750 Excitation Laser 405nm Violet Laser *Emission Filter / fluorochrome example* VL1: 450/40 \| Alexa Fluor 405, V450, BV421, Pacific Blue VL2: 512/25 \| Pacific Green VL3: 603/48 \| Pacific Orange, Qdot605 VL4: 710/50 \| Qdot705 Excitation Laser 561nm Yellow Laser *Emission Filter / fluorochrome example* YL1: 585/16 \| PE, PI YL2: 620/15 \| PE-Texas Red, PE-Alexa Fluor 610, 7-AAD YL3: 695/40 \| PE-Cy5.5, PE-Alexa Fluor 700 YL4: 780/60 \| PE-Cy7, Qdot800*	Info Reserve
BD FACSCalibur cell analyzer Reservable	Cell analyzer, Model: BD FACSCalibur, has two lasers (488nm Blue Laser, 633nm Red Laser). It has 4 fluorescence detectors. Excitation Laser 488nm Blue Laser *Emission Filter / fluorochrome example* A: 530/30 \| Alexa Fluor 488, FITC, BB515 B: 575/26 \| PE, PI, C: 670LP \| PerCP, PerCP-Cy5.5, BB700 Excitation Laser 633nm Red Laser *Emission Filter \| fluorochrome example* A: 661/16 \| APC, Alexa Fluor 647	Info Reserve
cell analyzer BD LSR II Reservable	Cell analyzer, Model: BD LSR II, has three lasers (488nm Blue Laser, 633nm Red Laser, and 405nm Violet Laser). It has 11 fluorescence detectors. Excitation Laser 488nm Blue Laser *Emission Filter \| fluorochrome example* A: 530/30 \| Alexa Fluor 488, FITC, BB515 B: 575/26 \| PE C: 610/20 \| PE-Texas Red D: 660/20 \| PE-Cy5 E: 695/40 \| PI, PerCP, PerCP-Cy5.5, BB700, 7-AAD F: 780/60 \| PE-Cy7 Excitation Laser 633nm Red Laser *Emission Filter / fluorochrome example* A: 670/30 \| APC, Alexa Fluor 647 B: 730/45 \| Alexa Fluor 700 C: 780/60 \| APC-Cy7, APC-H7 Excitation Laser 405nm Violet Laser *Emission Filter / fluorochrome example* A: 450/50 \| Alexa Fluor 405, V450, BV421, PB B: 525/50 \| Alexa Fluor 430, V500, BV510	Info Reserve
Entrust to use Reservable Entrusted	Accept the experimental commission of each flow cytometer model, or accompanying services and instrument certification services. Please contact with the manager after completing the timetable to discuss the experiment. Thanks Administrator: Ya-Fen Lu Miss ; Office phone: 03-2118800-5428 E-MAIL：yafenlu@mail.cgu.edu.tw	Info Reserve
Cytek Aurora Analyzer	Specifications: 5 lasers (355nm, 405nm, 488nm, 561nm, and 640nm) with 64 fluorescence channels Key Features: Detects over 40 colors in a single tube using highly similar dyes. Extracts multiple unique autofluorescence waveforms from complex samples to improve resolution. Uses "Cytek Assay Settings" for consistency and convenience. Dual systems: Switchable between "Full Spectrum Mode" and "Conventional Mode" Notice: Location: 6th Floor, Molecular and Clinical Immunology Center, First Medical Building For detailed regulations, please refer to the Instrument Usage Guidelines of the Molecular and Clinical Immunology Center Instrument Manager: Ms. You-Ning, Lin Tel: 03-2118800 ext. 3710 E-Mail: ynlin@cgu.edu.tw	Info Reserve
Octet RED96 Reservable	The biolayer interference technology BLI (BioLayer Interferometry) was used to carry out quantitative analysis of biomolecules and analysis of kinetics and affinity between biomolecules (ka, kd, KD). It can be used for the analysis of molecular interactions among antigen-antibody, nucleic acid-nucleic acid, drug-protein, nucleic acid-protein, protein-protein. Simultaneous determination of up to 8 channels. After the test, the sample can be recovered. Opening hours: Open from Monday to Friday, 08：00 to 16：30. For other hours, please contact the instrument administrator. Applicable consumables (provided by the user): Dip and ReadTM Biosensors Consumable reagents required for operation	Info Reserve
LionHeart FX Reservable	Automated camera system for cell fluorescence, bright field, high contrast bright field and color bright field. Performimage processing such as sitching, overlaying or background removal. Final analysis to get qualitative or quantitative data. Instrument Features: Automated microscope system for fluorescence, bright field, color bright field. Objective lens: 2.5X, 4X, 10X, 20X, 40X. Light source: high power LED, including DAPI filter cube, GFP filter cube, Texas Red filter cube, CY5 filter cube. Incubation temperature from room temperature to 40°C and CO2 gas controller. Gen5 software captures, processes, analyzes, and publishes images and data. Visit original website for application： https://www.biotek.tw/zh-TW/products/imaging-microscopy-automated-cell-imagers/lionheart-fx/ Opening hours： Opening hours are Monday to Friday, 08：00 to 16：30. For other hours, please contact the instrument administrator.	Info Reserve
Seahorse XF Pro Reservable	The Seahorse XF Pro utilizes a 96-well microplate format and label-free solid-state sensor technology to monitor cellular bioenergetics. By detecting real-time changes in oxygen and hydrogen ion concentrations in the extracellular microenvironment without direct contact with or damage to the cells, the instrument continuously measures the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR). These parameters provide dynamic insights into cellular energy metabolism, including mitochondrial respiration and glycolytic activity. Operating Hours Monday to Friday: 08：00–16：30 For instrument use outside regular operating hours, please contact the instrument manager in advance.	Info Reserve
EnSpire 2300 Reservable	Measurable category is ALPHA,Luminscence, Absorbance（260nm, 280nm, 405nm, 450nm, 540nm, 595nm）. Instrument Features : EnSpire Alpha 2300 can detect the fluorescent signal emitted by Alpha technology, and flexibly design experiments according to research needs. In addition to ALPHA, it can also detect cold and visible light (260nm, 280nm, 405nm, 450nm, 540nm, 595nm) Recent advances and application examples of this technology include: intermolecular interactions, target drug screening, cellular immunotherapy research, and enzyme activity analysis. It can also be used for analysis experiments such as cell proliferation and drug detection, reporter gene detection, etc. Opening hours: Open from Monday to Friday, 08：00 to 16：30.	Info Reserve