Instruments

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Octet RED96
Reservable
  • The biolayer interference technology BLI (BioLayer Interferometry) was used to carry out quantitative analysis of biomolecules and analysis of kinetics and affinity between biomolecules (ka, kd, KD). 
  • It can be used for the analysis of molecular interactions among antigen-antibody, nucleic acid-nucleic acid, drug-protein, nucleic acid-protein, protein-protein. 
  • Simultaneous determination of up to 8 channels. 
  • After the test, the sample can be recovered.

Opening hours: 

Open from Monday to Friday, 8:30 am to 5:00 pm. For other hours, please contact the instrument administrator.
 

Applicable consumables (provided by the user):

  • Dip and ReadTM Biosensors
  • Consumable reagents required for operation

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LionHeart FX
Reservable
Automated camera system for cell fluorescence, bright field, high contrast bright field and color bright field. Performimage processing such as sitching, overlaying or background removal. Final analysis to get qualitative or quantitative data.

Instrument Features:

  • Automated microscope system for fluorescence, bright field, color bright field. 
  • Objective lens: 2.5X, 4X, 10X, 20X, 40X. 
  • Light source: high power LED, including DAPI filter cube, GFP filter cube, Texas Red filter cube, CY5 filter cube. 
  • Incubation temperature from room temperature to 40°C and CO2 gas controller.
  • Gen5 software captures, processes, analyzes, and publishes images and data. 
  • Visit original website for application：
    https://www.biotek.tw/zh-TW/products/imaging-microscopy-automated-cell-imagers/lionheart-fx/

Opening hours：

Opening hours are Monday to Friday, 8:30 am to 5:00 pm. For other hours, please contact the instrument administrator. 
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INCell 1000
Reservable
Use the objective lens to take a large number of images with the automatic platform.
 1.Lamp：Xenon 100W
 2.Microscope：Nikon TE 2000
 3.Objective：
            4X/0.2、10X/0.45、
            20X/0.45、40X/0.6
 4.Laser autofocus
 5.CCD Camera
 6.Plate type：
            6-96 well plate and slide

Opening hours:

 Open from Monday to Friday, 8:30 am to 5:00 pm.
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EnSpire 2300
Reservable
Measurable category is ALPHA,Luminscence, Absorbance（260nm, 280nm, 405nm, 450nm, 540nm, 595nm）.

Instrument Features :

  • EnSpire Alpha 2300 can detect the fluorescent signal emitted by Alpha technology, and flexibly design experiments according to research needs.
  • In addition to ALPHA, it can also detect cold and visible light (260nm, 280nm, 405nm, 450nm, 540nm, 595nm)
  • Recent advances and application examples of this technology include: intermolecular interactions, target drug screening, cellular immunotherapy research, and enzyme activity analysis. It can also be used for analysis experiments such as cell proliferation and drug detection, reporter gene detection, etc.
 

Opening hours:

 Open from Monday to Friday, 8:30 am to 5:00 pm
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Investigator
Reservable
Identify fluorescent signal in the image(Tiff) to get quantity, fluorescence intensity, area, length and other value analysis results .

Opening hours:

Open from Monday to Friday, 8:30 am to 5:00 pm.
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Gen 5
Reservable
Perform image processing such as sitching, overlaying or background removal on images taken form LionHeart FX. Then analysis to get qualitative or quantitative data.

Opening hours:

Opening hours are Monday to Friday, 8:30 am to 5:00 pm. For other hours, please contact the instrument administrator.
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Centralized display
Reservable Entrusted
Commissioned to use GE INCell Analyzer 1000; GE Investigator; BioTek LionHeart FX; BioTek Gen 5. Other equipments after passing the education and training certification, use by userself.
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BD / FACSAriaIIu cell sorter
Reservable
Cell sorter, Model: BD / FACSAriaIIu, has four lasers (488nm Blue Laser, 633nm Red Laser, 375nm Near UV Laser and 405nm  Violet Laser ). It has 9 fluorescence detectors.

  • Excitation Laser  488nm  Blue Laser 
    • Emission Filter  | fluorochrome example
                          A: 530/30  |  Alexa Fluor 488, FITC, BB515
                          B: 575/26  |  PE
                          C: 610/20  |  PE-Texas Red
                          D: 695/40  |  PI, PerCP, PerCP-Cy5.5, BB700
                          E: 780/60  |  PE-Cy7

  • Excitation Laser  633nm  Red Laser 
    •  Emission Filter  | fluorochrome example
                          A: 660/20  |  APC, Alexa Fluor 647
                          B: 780/60  |  APC-Cy7, APC-H7

  •  Excitation Laser  375nm  Near UV Laser  ( 375nm or 405nm  choose one )
    •  Emission Filter  | fluorochrome example
                          A: 450/40  |  DAPI , Hoechst Blue (Cell cycle)
                          B: 670LP   |  Hoechst Red (Side population)

  •  Excitation Laser  405nm  Violet Laser  ( 375nm or 405nm  choose one )
    •  Emission Filter  | fluorochrome example
                        A: 450/40  |  Alexa Fluor 405, V450, BV421, PB
                        B: 510/50  |  Alexa Fluor 430, V500, BV510

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Thermo Attune NxT cell analysis
Reservable
Cell analysis, Model: Thermo Attune NxT, has four lasers (488nm Blue Laser, 633nm Red Laser, 405nm Violet Laser and 561nm Yellow Laser). It has 14 fluorescence detectors.

  • Excitation Laser  488nm  Blue Laser 
    • Emission Filter  | fluorochrome example
                        BL1: 530/30  |  Alexa Fluor 488, FITC, BB515
                       BL2: 575/26  |  PE-Alexa Fluor 610, PE, PE-Texas Red
                       BL3: 695/40  |  PerCP, PE-Cy5.5, PerCP,-Cy5.5, BB700
                  
  • Excitation Laser  637nm  Red Laser 
    •  Emission Filter  | fluorochrome example
                        RL1: 670/14  |  APC, Alexa Fluor 647
                       RL2: 720/30  |  Alexa Fluor 680, APC-Alexa Fluor 700
                       RL3: 780/60  |  APC-Cy7, APC-Alexa Fluor 750

  •  Excitation Laser  405nm  Violet Laser  
    •  Emission Filter  | fluorochrome example
                        VL1: 450/40  |  Alexa Fluor 405, V450, BV421, Pacific Blue
                        VL2: 512/25  |  Pacific Green
                        VL3: 603/48  |  Pacific Orange, Qdot*605
                        VL4: 710/50  |  Qdot*705

  •  Excitation Laser  561nm  Yellow Laser  
    •  Emission Filter  | fluorochrome example
                        YL1: 585/16  |  PE, PI
                        YL2: 620/15  |  PE-Texas Red, PE-Alexa Fluor 610, 7-AAD
                        YL3: 695/40  |  PE-Cy5.5, PE-Alexa Fluor 700
                        YL4: 780/60  |  PE-Cy7, Qdot*800

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BD FACSCalibur cell analysis
Reservable
Cell analysis, Model: FACSCalibur, has two lasers (488nm Blue Laser, 633nm Red Laser). It has 4 fluorescence detectors.

  • Excitation Laser  488nm  Blue Laser 
    • Emission Filter  | fluorochrome example
                         A: 530/30  |  Alexa Fluor 488, FITC, BB515
                         B: 575/26  |  PE, PI,
                         C: 670LP   |  PerCP, PerCP-Cy5.5, BB700
                  
  • Excitation Laser  633nm  Red Laser 
    •  Emission Filter  | fluorochrome example
                          A: 661/16  |  APC, Alexa Fluor 647


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cell analysis BD LSR II
Reservable
Cell analysis, Model: BD LSR II, has three lasers (488nm Blue Laser, 633nm Red Laser, and 405nm Violet Laser). It has 11 fluorescence detectors.

  • Excitation Laser  488nm  Blue Laser 
    • Emission Filter  | fluorochrome example
                         A: 530/30  |  Alexa Fluor 488, FITC, BB515
                         B: 575/26  |  PE
                         C: 610/20  |  PE-Texas Red
                         D: 660/20  |  PE-Cy5
                         E: 695/40  |  PI, PerCP, PerCP-Cy5.5, BB700, 7-AAD
                         F: 780/60  |  PE-Cy7

  • Excitation Laser  633nm  Red Laser 
    •  Emission Filter  | fluorochrome example
                           A: 670/30  |  APC, Alexa Fluor 647
                          B: 730/45  |  Alexa Fluor 700
                         C: 780/60  |  APC-Cy7, APC-H7

  •  Excitation Laser  405nm  Violet Laser  
    •  Emission Filter  | fluorochrome example
                          A: 450/50  |  Alexa Fluor 405, V450, BV421, PB
                         B: 525/50  |  Alexa Fluor 430, V500, BV510

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Entrust to use
Reservable Entrusted
Accept the experimental commission of each flow cytometer model, or accompanying services and instrument certification services. Please contact with the manager after completing the timetable to discuss the experiment. Thanks

Administrator: 
  • Ya-Fen Lu Miss ; Office phone: 03-2118800-5428
  • E-MAIL：yafenlu@mail.cgu.edu.tw

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Autoclave 733HC & 733HC-E
Reservable 
Handling infectious waste

Opening hours:

From 8:00 am every Monday, the reservation is open from Monday to Friday,
divided into three periods: 9:30, 12:30 and 14:30.
Please arrive 10 minutes before the reserved time slot.
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