Instruments

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BD FACSAriaIIu cell sorter
Reservable
Cell sorter, Model: BD FACSAriaIIu, has four lasers (488nm Blue Laser, 633nm Red Laser, 375nm Near UV Laser and 405nm  Violet Laser ). It has 9 fluorescence detectors.

  • Excitation Laser  488nm  Blue Laser 
  • Emission Filter  /  fluorochrome example
                          A: 530/30  |  Alexa Fluor 488, FITC, BB515
                          B: 575/26  |  PE
                          C: 610/20  |  PE-Texas Red
                          D: 695/40  |  PI, PerCP, PerCP-Cy5.5, BB700
                          E: 780/60  |  PE-Cy7

  • Excitation Laser  633nm  Red Laser 
  •  Emission Filter  /  fluorochrome example
                          A: 660/20  |  APC, Alexa Fluor 647
                          B: 780/60  |  APC-Cy7, APC-H7

  •  Excitation Laser  375nm  Near UV Laser  ( 375nm or 405nm  choose one )
    •  Emission Filter  | fluorochrome example
                          A: 450/40  |  DAPI , Hoechst Blue (Cell cycle)
                          B: 670LP   |  Hoechst Red (Side population)

  •  Excitation Laser  405nm  Violet Laser  ( 375nm or 405nm laser only choose one )
  •  Emission Filter  /  fluorochrome example
                        A: 450/40  |  Alexa Fluor 405, V450, BV421, PB
                        B: 510/50  |  Alexa Fluor 430, V500, BV510

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Thermo Attune NxT cell analyzer ( 637nm laser error, can't use APC,APC-Cy7 etc dye ) )
Reservable
Cell analyzer, Model: ThermoFisher Attune NxT, has four lasers (488nm Blue Laser, 633nm Red Laser, 405nm Violet Laser and 561nm Yellow Laser). It has 14 fluorescence detectors.

  • Excitation Laser  488nm  Blue Laser 
  • Emission Filter  |  fluorochrome example
                     BL1: 530/30  |  Alexa Fluor 488, FITC, BB515
                     BL2: 575/26  |  PE-Alexa Fluor 610, PE, PE-Texas Red
                     BL3: 695/40  |  PerCP, PE-Cy5.5, PerCP,-Cy5.5, BB700
                  
  • Excitation Laser  637nm  Red Laser 
  • Emission Filter  /  fluorochrome example
                     RL1: 670/14  |  APC, Alexa Fluor 647
                     RL2: 720/30  |  Alexa Fluor 680, APC-Alexa Fluor 700
                     RL3: 780/60  |  APC-Cy7, APC-Alexa Fluor 750

  •  Excitation Laser  405nm  Violet Laser  
  •  Emission Filter  /  fluorochrome example
                     VL1: 450/40  |  Alexa Fluor 405, V450, BV421, Pacific Blue
                     VL2: 512/25  |  Pacific Green
                     VL3: 603/48  |  Pacific Orange, Qdot*605
                     VL4: 710/50  |  Qdot*705

  •  Excitation Laser  561nm  Yellow Laser  
  •  Emission Filter  /  fluorochrome example
                     YL1: 585/16  |  PE, PI
                     YL2: 620/15  |  PE-Texas Red, PE-Alexa Fluor 610, 7-AAD
                     YL3: 695/40  |  PE-Cy5.5, PE-Alexa Fluor 700
                     YL4: 780/60  |  PE-Cy7, Qdot*800

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BD FACSCalibur cell analyzer
Reservable
Cell analyzer, Model: BD FACSCalibur, has two lasers (488nm Blue Laser, 633nm Red Laser). It has 4 fluorescence detectors.

  • Excitation Laser  488nm  Blue Laser 
  • Emission Filter  /  fluorochrome example
                     A: 530/30  |  Alexa Fluor 488, FITC, BB515
                     B: 575/26  |  PE, PI,
                     C: 670LP   |  PerCP, PerCP-Cy5.5, BB700
                  
  • Excitation Laser  633nm  Red Laser 
  • Emission Filter  |  fluorochrome example
                    A: 661/16  |  APC, Alexa Fluor 647


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cell analyzer BD LSR II
Reservable
Cell analyzer, Model: BD LSR II, has three lasers (488nm Blue Laser, 633nm Red Laser, and 405nm Violet Laser). It has 11 fluorescence detectors.

  • Excitation Laser  488nm  Blue Laser 
  • Emission Filter  |  fluorochrome example
                     A: 530/30  |  Alexa Fluor 488, FITC, BB515
                     B: 575/26  |  PE
                     C: 610/20  |  PE-Texas Red
                     D: 660/20  |  PE-Cy5
                     E: 695/40  |  PI, PerCP, PerCP-Cy5.5, BB700, 7-AAD
                     F: 780/60  |  PE-Cy7

  • Excitation Laser  633nm  Red Laser 
  • Emission Filter  /  fluorochrome example
                     A: 670/30  |  APC, Alexa Fluor 647
                     B: 730/45  |  Alexa Fluor 700
                     C: 780/60  |  APC-Cy7, APC-H7

  •  Excitation Laser  405nm  Violet Laser  
  •  Emission Filter  /  fluorochrome example
                      A: 450/50  |  Alexa Fluor 405, V450, BV421, PB
                      B: 525/50  |  Alexa Fluor 430, V500, BV510

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Entrust to use
Reservable Entrusted
Accept the experimental commission of each flow cytometer model, or accompanying services and instrument certification services. Please contact with the manager after completing the timetable to discuss the experiment. Thanks

Administrator: 

  • Ya-Fen Lu Miss ; Office phone: 03-2118800-5428
  • E-MAIL：yafenlu@mail.cgu.edu.tw

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Cytek Aurora Analyzer

Specifications: 5 lasers (355nm, 405nm, 488nm, 561nm, and 640nm) with 64 fluorescence channels


Key Features:

  • Detects over 40 colors in a single tube using highly similar dyes.

  • Extracts multiple unique autofluorescence waveforms from complex samples to improve resolution.

  • Uses "Cytek Assay Settings" for consistency and convenience.

  •  Dual systems: Switchable between "Full Spectrum Mode" and "Conventional Mode"

Notice:

  • Location: 6th Floor, Molecular and Clinical Immunology Center, First Medical Building

  • For detailed regulations, please refer to the Instrument Usage Guidelines of the Molecular and Clinical Immunology Center

Instrument Manager:

  • Ms. You-Ning, Lin
  • Tel: 03-2118800 ext. 3710
  • E-Mail: ynlin@cgu.edu.tw

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